uSocial CEO Leon Hill said Twitter used a "brand-management organisation" to accuse the company of "spamming".
"The definition of spam is using electronic messaging to send unsolicited communication and as we don't use Twitter for this, the claims are false," said Hill.
"The people at Twitter who are sending these claims are just flailing around trying to look for any excuse they can, though it's going to take much more than this if they want us to pack up shop."
Twitter has yet to respond to a request for comment on the matter.
See also: Twitter finally embraces re-tweeting