You have the remains of the virus still present. It is clearly looking to run at start up but can not find it's associated program.
At Start > Run type msconfig and look at the 'Startup' tab. Look through the list and see if anything seems to be associated with this message. If it does then remove the tick, restart the machine and see if you get the message.
You could also run CCCleaner click here and / or Regseeker click here to remove redundant registry entries.