It shold work for all most all cases, no matter 2k or XP. But, uninstall motherboard driver for old board before swap in case any conflict. Once you boot up with new board, install driver for new motherbaord.
Even if it does not work, you can fix it. Put Win2k or XP CD in CD-ROM, restart PC and make it boot up from CD. When you see menu of option, select Recovery Consonl, then just type "fixboot" it will fix it. Still not work, type "help" under console, you can find a lot of commands to try.
For XP, you need to reactivate with Microsoft if your version is OEM or Retail version. If your version is large volumn coorporation version, then no reactivative is needed.