If I understand you correctly, you have one hard drive with two partitions, C: and E:, and you are trying to backup the C: partition to E: but cannot verify the saved file.
Is there any other physical disc you can get hold of to which you can try and back up? Borrow an external HDD for example? This would eliminate any possible physical damage to that portion of the existing disc upon which the E: partition resides causing corruption. When you re-formatted the E: partition, did you do a surface scan?
Otherwise it could be that Acronis is misreporting the failed validation for some reason. At this point I'm lost....
As an afterthought, (and I don't mean to be rude), is there enough room in the E: partition for the .tib file? I don't know what happens if there isn't.